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As part of a multiyear project to promote a cooperative relationship between U.S. and Pakistani human and animal health and infectious disease experts, the Pakistan Academy of Sciences, together with the U.S. National Academies of Sciences, Engineering, and Medicine, convened a bilateral workshop in Islamabad, Pakistan, to promote best practices in and improved communications, cooperation, and coordination among public, private, military, and animal health clinical laboratories in Pakistan. The workshop, "Strengthening and Sustaining a Network of Public and Animal Health Clinical Laboratories in Pakistan," was held on September 27-29, 2016. Pakistani life science, public health, veterinary, and clinical laboratory experts, graduate students from Pakistani institutions of higher learning, and U.S. scientists/clinicians met at the workshop to explore questions facing human and animal health policy makers in Pakistan.This publication summarizes presentations and discussions of the workshop.