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The Present book deals with the Biotechnological research work on the medicinally important species Solanum surattense. This book has been written for researchers those who are doing research for improvement of medicinal plants using biotechnological tools. The subject matter has been presented in lucid manner with the help of photos figures and tables. It covers the review of plant tissue culture in general including the species callus induction from different explants and callus mediated and diiect plant regeneration induction of somatic enbryogenesis and plantlet formation micropropagation (shoot tip and nodal culture) plantlet formation from floral bud culture haploid plant production salt and drought tolerant cell lines selection development of antibiotic resistance and Agrobacterium mediated genetic transformation. Each chapter has been thoroughly reviewed. This book will be useful for researchers of Plant Science Agriculture Pharmacy Plant Biotechnology and Ayurvedic Medicine.